There are two versions of them. The first version of the Beagle Babes appear only in the comic books as the daughters or nieces of the Beagle Boys. They are often used as common enemies of the Junior Chickadees.
The second version of the Beagle Babes appear only in the Duck Tales episode The Good Muddahs where they are known to be female cousins of the Beagle Boys. In that episode they kidnap Webby Vanderquack (Mrs. Bentina Beakly's granddaughter and Scrooge McDuck's adopted grandniece).