It can be produced by starting on a high note and playing the fourth below or by starting on a low note and playing the fourth above.

A perfect fourth in just intonation corresponds to a pitch ratio of 3:4 or 1:1.333...while in an equal tempered tuning, a perfect fourth is equal to five semitones, a ratio of 1:2^{5/12} (approximately 1:1.3348), or 500 cents, about 1.955 centss wide.

The perfect fourth is considered the most consonant interval after the unison, octave, and perfect fifth.

Conventionally, the strings of a double bass and a bass guitar are tuned by intervals of perfect fourths, while all strings but one of a guitar are tuned to intervals of perfect fourths.