The experiment was a simple one in concept. Bacteria are able to transfer genetic material through a liquid medium to other bacteria, transforming (giving new genetic characteristics) them. Therefore if bacteria transform when an organic molecule is placed in liquid with them, then that molecule holds genetic information. For the experiment, a bacteria called a pneumococcus was used. The R strain is harmless, while the S strain causes pneumonia in laboratory mice.
Substances were available to remove various organic compounds from bacteria, and if the remaining organic compounds were still able to cause R strain bacteria to transform then the substances removed couldn't be the carrier of genes. S strain bacteria first had the large cellular structures removed. Then they were treated with proteases, which removed the proteins from the cells before the remainder was placed with R strain bacteria. The R strain bacteria transformed, meaning that proteins didn't carry the genes for causing the disease. Then the remnants of the S strain bacteria were treated with a deoxyribonuclease which removed the DNA. After this treatment, the R strain bacteria no longer transformed, as evidenced by mice not showing signs of pneumonia when injected with the bacteria. This indicated that DNA was the carrier of genes in cells.
Avery wrote in a letter to his brother, "It's lots of fun to blow bubbles but it's wiser to prick them yourself before someone else tries to."
Nobel laureate Joshua Lederburg stated that Avery and his laboratory provided "the historical platform of modern DNA research" and "betokened the molecular revolution in genetics and biomedical science generally."