(bovine γ chymotrypsin: PDB 1AB9
, EC 220.127.116.11) is a digestive enzyme that can perform proteolysis
Activation of chymotrypsin
Chymotrypsin is synthesized by protein biosynthesis
as a precursor called chymotrypsinogen
that is enzymatically inactive. On cleavage by trypsin
into two parts that are still connected via an S-S bond, cleaved chymotrypsinogen molecules can activate each other by removing two small peptides in a trans
-proteolysis. The resulting molecule is chymotrypsin, three polypeptides interconnected via S-S bonds.